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Sftpc creert2

WebUsing Sftpc CreERT2/+ : tdTomato flox/flox mice, we previously identified a lung populat... View Identification of a novel subset of alveolar type 2 cells enriched in PD-L1 and … Web28 May 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell preparation, lungs were inflated intratracheally with 3 mL dispase (5 U/mL; BD Biosciences #354235) and for further digestion, each lung was incubated in 3 mL dispase solution (5 U/mL) at room …

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WebCx3cr1 CreERT2 and Sall1 CreERT2 mice were crossed to two reporter strains: R26-CAG-ZsGreen and R26-YFP (Fig. 1A). In R26-CAG-ZsGreen mice, the reporter construct consists … Web10 May 2024 · Interestingly, AT2 cell proliferation at P5 occurred primarily in AT2 cells lacking the YFP lineage mark from SftpcCreERT2;R26REYFP mice ( Figures 2 D and 2E). To determine the response of AT1 cells during this injury, we lineage traced AT1 cells during hyperoxia ( Figure 2 G). matt ormond bremerton wa https://reospecialistgroup.com

Regeneration of the lung alveolus by an evolutionarily ... - Nature

Web30 Mar 2024 · (A) A representative FACS gating strategy used in this study to isolate Hopx + AT1 and Sftpc + AT2 cells from SftpcEGFP, SftpcCreERT2; R26REYFP, HopxEGFP, and HopxCreERT2; R26REYFP mice. The EGFP channel voltage is lower for SftpcEGFP mice. Web21 Sep 2024 · Surprisingly, when Tmx was injected after viral infection, we observed 7.9% ± 1.4% Trp63 CreERT2 lineage-labeled cells expressed the AT2 cell marker SftpC but not p63 or Krt5 in the areas that surrounded the injured foci, and the number was increased to 19.4%±4.2% in Trp63 CreERT2;Trpm5-/-;R26 Ai14 mice (Figure 5A and Figure 5—figure ... WebMouse Lines Sftpc Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … matt osberg helen of troy

Mitofusins regulate lipid metabolism to mediate the

Category:FGF10-FGFR2B Signaling Generates Basal Cells and Drives …

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Sftpc creert2

Age-dependent alveolar epithelial plasticity orchestrates lung ...

Web12 Jul 2024 · Immunofluorescence staining of the lung showed that the HGF receptor Met was expressed almost exclusively in the SFTPC + distal alveolar stem cells that were adjacent to Gli2 + distal mesenchyme in vivo ( Figure 4F ), suggesting that Hh activation in the distal alveolar mesenchyme might regulate HGF activation of MET in the alveolar … http://www.informatics.jax.org/disease/DOID:1324

Sftpc creert2

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WebUsing the Sftpc CreERT2; tdTomato lineage-traced mice, we recently reported a novel AT2-IAAP subpopulation characterized by low Sftpc expression but that is enriched for PD-L1 expression . IAAP cells, which represent quiescent and immature epithelial progenitors, undergo activation and expansion following PNX injury . WebSPC-Cre-ERT2 transgenic mouse for temporal gene deletion in alveolar epithelial cells Although several Cre-loxP-based gene knockout mouse models have been generated for …

Web28 Mar 2024 · Mice were maintained under specific pathogen-free conditions. The Kras LSL, Etv5 fl, Trp53 fl, Rosa26 CreERT2, Rosa26 tdTomato, and Sftpc CreERT2 alleles were … Web1 Feb 2024 · SFTPC-CreERT2;R26ReYFP at 7 days of culture. Membrane in magenta, SFTPC in green and nuclei in grey. Why I like this preprint. 3D cultures of AT2 cells are currently being used more and more for disease modeling of lung diseases involving the alveoli. From lung adenocarcinoma to COVID, there are multiple deadly diseases that use AT2 ...

Web3 Aug 2024 · including our recently identified AT2-SftpcLow (tdTomLow, aka injury-activated alveolar progenitors, IAAPs) [22]. In contrast to mature AT2-SftpcHigh …

WebMutations in human and/or mouse homologs are associated with this disease. Synonyms: lung neoplasm matt orlando bowdoin collegeWebCx3cr1 CreERT2 and Sall1 CreERT2 mice were crossed to two reporter strains: R26-CAG-ZsGreen and R26-YFP (Fig. 1A). In R26-CAG-ZsGreen mice, the reporter construct consists of the ZsGreen sequence placed downstream of three floxed SV40 polyA sequences (0.9 kb) that block transcription from the strong artificial CAG promotor 12. matt ory attorney thibodauxWeb15 Jun 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell preparation, lungs were inflated intratracheally with 3 mL dispase (5 U/mL; BD Biosciences #354235) and for further digestion, each lung was incubated in 3 mL dispase solution (5 U/mL) at room … matt or silk paint for wallsWeb10 May 2024 · Citation: Ahmadvand, N.; Carraro, G.; Jones, M.R.; Shalashova, I.; Noori, A.; Wilhelm, J.; Baal, N.; Khosravi, F.; Chen, C.; Zhang, J.-S.; et al. Cell-Surface ... matt orthodonticsWeb6 Feb 2024 · However, tamoxifen-treated Sftpc CreERT2; hACE2 fl/y males infected with SARS-CoV-2 virus exhibited levels of viral nucleocapsid protein and viral RNA in the lung indistinguishable from those in hACE2 fl/y animals (Fig 3B and 3C). These findings suggested that cell types other than AT2 cells can support SARS-CoV-2 infection of the lung. mat torslandaWeb19 Oct 2024 · The Sftpc-CreERT2 mouse line has been used previously to track AT2 cell fate (65, 66). Gramd2-CreERT2 mice were generated by homologous recombination insertion of a cassette including CreERT2- downstream of … matt osborne lawyerWeb19 Feb 2024 · To assess this, we determined the relative contribution of AT1 and AT2 progenitor cells to the mature AT1 and AT2 lineages by using established AT1- and AT2-specific promoter-driven inducible Cre recombinases, HopxCreERTand SftpcCreERT2(23–27). matt osborn cornerstone