Web15 jan. 2004 · Protonema cells were grown on BCD medium supplemented with 1 mM CaCl 2, 5 mM ammonium tartrate, and 0.5% (w/v) glucose (BCDATG medium, Nishiyama et al. 2000); the cells were collected every 5 d, and were ground with a homogenizer (Physcotron, Microtec Nition, Chiba) before they were applied to a new BCDATG agar … Webmechanically disintegrated using a Physcotron NS-56 (Microtec, Japan) equipped with a 20 mm-diameter shaft at 7500 rpm for 6 min, and sonicated using a US-300T (Nihon Seiki, Japan) equipped with a 26 mm-diameter tip at 70% output for 8 min. The unfibrillated fraction (~30%) was separated by centrifugation at

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http://www.greatfallsdogs.com/enarch1658158.html Webized in 70% (v/v) MeOH (6L) with a Physcotron ultra-homogenizer (Microtec). The homogenate was shaken overnight with a shaker and then filtered to obtain an extract. … roots and shoots haddington

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WebMicroTec - Homogenizer Available in various types: Universal-type hom... WebMicroTec - Homogenizer. Available in various types: Universal-type homogenizer, ultrasonic homogenizer, and handy micro homogenizer (for a small amount of sample) Your … Web17 jun. 2014 · After the pH was stabilized, the reaction lasted for 2 h. In the second step, the pH was adjusted to 7 by adding 0.5 M HCl. The mixture was then filtered and washed thoroughly with water. Finally, 0.45 g of TOCN was suspended in deionized water (100 mL) and treated with a double-cylinder-type homogenizer (Physcotron NS-56; Microtec) for … roots and shoots east lothian