WebDec 5, 2006 · To analyze expression of mouse and rat exons I–IV-, exon VI-, and exon IXA-specific transcripts, cDNA was amplified in a total volume of 25 μl with 35 cycles of PCR using HotFire polymerase system (Solis BioDyne, Estonia). An annealing temperature of 60°C was used for all primer combinations. WebJul 29, 2015 · A new DNA aptamer targeting Protein A is presented. The aptamer was selected by use of the FluMag-SELEX procedure. The SELEX technology (Systematic Evolution of Ligands by EXponential enrichment) is widely applied as an in vitro selection and amplification method to generate target-specific aptamers and exists in various …
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WebThe enzyme blend has 5’→ 3’ exonuclease activity and 3’→ 5’ proofreading activity for increased copy fidelity. This results in an increased fidelity (up to five fold) compared to … WebSolis BioDyne OÜ Address: Teaduspargi 9, 50411 Tartu, Estonia Phone: +372 740 9960 Fax: +372 740 2079 Email: [email protected] SKYPE: solis.biodyne taco time white chicken chili nutrition
BAC-based cellular model for screening regulators of BDNF gene ...
WebSMITHSONIAN @& ROGER BRIDGMAN SMITHSONIAN &€3 ta ~~ Os “Oo J ( 2 . \ i UF D ~ ~ Ww . if P ve oa INVENTIONS AND DISCOVERIES Written by Roger Bridgman A Dorling Kindersley Boo WebAug 12, 2013 · PCR was carried out in 25 µl using 5 U HotFire polymerase (Solis Biodyne, Tartu, Estonia), 1.5 mM MgCl 2, 80 µM dNTPs, 200 nM specific primers, with the reverse primers carrying a biotin tag, and 1 µl of DNA template. Primer sequences are listed in … WebPCR set-up. Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA must be released from the bacteria in order to serve as PCR template. Dealing with this and a few … taco time white chicken chili