WebOct 7, 2016 · Stable FHCC98 cells expressing forced miR-129-5p sponge were established. HepG2 cells stably expressing Pri-miR-129-2 with mutated seed region were also … WebThomas Jefferson University Jefferson Digital Commons Department of Emergency Medicine Faculty Papers Department of Emergency Medicine 1-1-2009
miR-148a is downregulated in HCC, and low expression predicts …
WebJul 1, 2024 · The following human liver cancer cell lines were used: (a) SMCC7721 and Hep3B cells were kindly provided by Dr. Zhiyong Mao (Tongji University), (b) HCCLM9 … WebMethods: Human SMMC-7721 and FHCC98 cells were cultured and transfected with siRNA fragments against HAb18G/CD147. The expression levels of HAb18G/CD147 and integrin α6β1 were determined by immunofluorescent double-staining and confocal imaging analysis. Co-immunoprecipitation and road trip when pregnant
RESEARCH Open Access Overexpression of brachyury …
WebJun 1, 2013 · c‐Myc (Myc) plays an important role in normal liver development and tumorigenesis. We show here that Myc is pathologically activated in and essential for promoting human hepatocellular carcinoma (HCC). Myc induces HCC through a novel, microRNA (miRNA)‐mediated feedback loop comprised of miR‐148a‐5p, miR‐363‐3p, … WebFeb 6, 2013 · Human liver tumor-derived cell lines used in this study—including HepG2, Bel-7402, FHCC98, and Huh-7—were cultured under standard cell culture conditions in Dulbecco's modified Eagle's medium (DMEM) (GIBCO, Life Technologies) supplemented with 10% fetal bovine serum (GIBCO), 1% L -glutamine, 1% penicillin-streptomycin, and … WebJul 13, 2024 · To investigate the role of miR-148a on tumor growth, an orthotopic liver cancer model was used. As shown in Supplementary Figure 2b and c, the tumors formed by miR-148a-3p sponge-expressing FHCC98 cells were bigger than those formed by control FHCC98 cells. In contrast, tumors formed by Pri-miR-148a expressing HepG2 cells were … road trip where to watch